M13 phage DNA probe informativity rising at studying pig genome
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چکیده
منابع مشابه
Improved method for the production of M13 phage and single-stranded DNA for DNA sequencing.
An improved method is described for the efficient production of M13 phage and M13 single-stranded (ss)DNA in a relatively short time period. Infection of E. coli (F') cells with as few as 5 phage particles can yield 10(12) phage particles/mL in 3 hours if the cells are grown in LB broth or SOB broth supplemented with about 5 mM Mg2+. The method tolerates large variations in the initial multipli...
متن کاملOligopeptide M13 Phage Display in Pathogen Research
Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in...
متن کاملStability of M13 Phage in Organic Solvents
The effect of different types of organic solvents on the structural integrity of M13 phages has been directly visualized by transmission and scanning electron microscopy. The exposure of M13 phages to apolar hexane had no effect on the structure of the phages for up to 8 h. In contrast, phages showed ~10-fold contraction into rod-like I-forms and to flattened spheroids with ~12 nm diameter upon...
متن کاملGenome-wide comparison of phage M13-infected vs. uninfected Escherichia coli.
To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated ...
متن کاملA simple non-isotopic method for DNA fingerprinting with M13 phage.
Recently it was discovered that the bacteriophage M13 contains in its protein III gene, tandem repeats of a 15-bp motif that can recognize a family of hypervariable minisatellites in vertebrate DNA (1). Southern blots of human DNA digested with Hae III and probed with 32p_iaDeled M13 revealed complex individual-specific DNA fingerprints, similar to those described by Jeffreys et al (2) with pro...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1996
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.00042f